- Neutralize the activity of the target antibody by blocking the antibody’s ligand binding site
- Quantitate the antibody in serum or expression system supernatants
- Occasionally anti-ID mAbs will be a mirror image of ligands for the idiotype.
Our approach is to immunize with the purified antibody (or Fab fragment) and to screen against the antigen IgG and an irrelevant IgG as a control. Antibodies (anti-ID) with the correct specificity are expanded. Specificity to the variable region is confirmed using the ligand recognized by the antigen antibody.