Rat Monoclonal Antibodies: Custom Development
- Used for murine antigens.
- Reduced antigen requirement. Single rat immunized versus multiple mice.
- Host variation offers different antibody responses.
- Larger spleens yield twice the number of hybridomas per fusion.
- No special growth media required.
- Purification methods similar for murine antibodies
- Pair with existing mouse antibodies in sandwich assays
Schedule for a typical rat hybridoma project:
Phase I: Immunization (4-6 weeks)
Approximately 0.5-1.5 mg of protein is required for standard immunizations. A serum titer will determine when we schedule a fusion.
Phase II: Fusion (2 weeks from fusion to screening)
At this stage, splenocytes are plated into either 32 or 64 x 96 well plates, and screened by solid-phase ELISA for antigen specific clones. Fusion positives are expanded into 24-well plates and can be rescreened using a number of different assays that will help you identify the most desirable clones for your needs.
Phase III: Subcloning (2 weeks)
Fusion positive clones are subcloned by limiting dilution and screened for antigen specific positive clones by solid-phase ELISA.
Phase IV: Characterization and purification
We offer a wide variety of services including antibody production and purification, antibody characterization, and cell line archival. Please contact us for more information.
- Aggregation Analysis Using Light Scattering
- ELISA Assay Development
- Large Scale Antibody Production and Purification