Repetitive Immunizations Multiple Sites (RIMMS)
Many variables determine the specificity, affinity, isotype, and number of distinct monoclonal antibodies you obtain following somatic cell fusion of antibody-producing splenocytes and myeloma cells. Investigators need high affinity antibodies and oftentimes large numbers of distinct specificities, so immunization protocols tend to run weeks or months. These longer protocols typically require up to a milligram of antigen to immunize several animals multiple times over several months. Fusion only occurs when there is a high titer serum immune response.As an alternative to our standard immunization protocol, the Repetitive Immunizations Multiple Sites (RIMMS) strategy uses lymphocytes from regional draining lymph nodes and a significantly shorter immunization schedule: two weeks instead of several months. Along with using less antigen, this approach allows the investigator to obtain hybridomas in a month.RIMMS is an ideal strategy when you need one or several monoclonal antibodies quickly. The standard immunization protocol is recommended when you need large numbers of distinct monoclonal antibodies.
- Requires less antigen for immunization
- Can produce positive hybridoma antibodies in 4 weeks
- Useful when an antibody is needed quickly
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2. Kilpatrick, K.E., et al. (1997) Hybridoma 16, 381-389