Aggregation Analysis Using Light Scattering
“There is no more fundamental means for measuring the mass of molecules than light scattering. It is a time-proven and absolute procedure that should be included among the tools of every physical, polymer, or biological scientist.” — Philip J. Wyatt, Analytica Chimica Acta, 272 (1993) 1-40.
Light scattering is a rapid non-destructive method for studying motions of macromolecules in solution and cells in suspension. For proteins such as monoclonal antibodies, dynamic light scattering measures the diffusion and size of the antibodies while static light scattering reports the molecular weight and radius of gyration. By knowing that your purified antibody is in solution following purification you can eliminate one more problem variable in understanding why a particular antibody “doesn’t work” in a particular assay. And if the antibody does aggregate, light scattering is a tool for determining alternative solution conditions to improve its behavior in solution.
It won’t work if it’s out of solution. — Bill Church, unpublished.