mAb-Antigen Affinity Using Octet

The Octet system by ForteBio uses Bio-Layer Interferometry (BLI), a label-free method for measuring bi-molecular interactions. With Dip and Read technology there is no need to purify antibodies to get valuable antigen binding and specificity information.Please contact us to discuss how BLI can help you select the right antibody.

Options

Octet Biosensors come in a variety of surface chemistries useful for designing an assay to suit your needs.

Some examples are:

  • Streptavidin
  • Protein G
  • Protein L
  • Anti-human IgG Fc
  • Anti-murine IgG Fc
  • Anti-GST
  • Anti-Penta-HIS

Find out more about the Octet system

Uses

  • Early stage screening of hybridomas –identify high affinity hybridomas before the subcloning stage
  • Quantitate antibody in bioprocessing – On rates are proportional to antibody concentration
  • Kinetic analysis of antibodies – on and off rates and apparent Kds determined in minutes, label free
  • Epitope mapping –identify antibodies that bind to overlapping or spacially distinct epitopes
  • Antibody pair selection – identify possible capture and detector antibodies for sandwich or quantitative ELISAs
  • Detect and quantitate proteins in serum – useful for pharmakokinetics and immunogenicity studies

Advantages

  • Label-free detection system
  • Process 8 assays in parallel
  • Intuitive, real-time format
  • Reduces the time to create new binding assays