Scientists at GMA Collaborate on Bovine Cytokine Project

Scientists at Green Mountain Antibodies have recently collaborated with the USDA and the University of Vermont to produce novel murine monoclonal antibodies to bovine cytokines IL-13 and IL-17A. IL-13 induces B-cell activation and proliferation and possesses anti-inflammatory properties, while IL-17A is primarily responsible for the proinflammatory mechanisms of chronic inflammatory states. Monoclonal antibodies were generated using DNA immunization of mice with adenovirus5 vector containing cytokine inserts; use of adenovirus allowed the same virus construct for generating an immune response and for cytokine expression in cell culture. Hybridomas secreting anti-cytokine antibodies were selected by solid-phase ELISA using purified recombinant cytokines containing FLAG tags. Antibodies that bound cytokine without FLAG were characterized by western blotting, ELISA, and fluorescence-activated cell sorting of bovine lymphocytes. These findings were presented at the International Veterinary Immunology Symposium in Australia. Anti-cytokine monoclonal antibodies are useful in measuring cellular immune correlates of vaccination in livestock.


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