Scientists at Green Mountain Antibodies have recently collaborated with the USDA and the University of Vermont to produce novel murine monoclonal antibodies to bovine cytokines IL-13 and IL-17A. IL-13 induces B-cell activation and proliferation and possesses anti-inflammatory properties, while IL-17A is primarily responsible for the proinflammatory mechanisms of chronic inflammatory states. Monoclonal antibodies were generated using DNA immunization of mice with adenovirus5 vector containing cytokine inserts; use of adenovirus allowed the same virus construct for generating an immune response and for cytokine expression in cell culture. Hybridomas secreting anti-cytokine antibodies were selected by solid-phase ELISA using purified recombinant cytokines containing FLAG tags. Antibodies that bound cytokine without FLAG were characterized by western blotting, ELISA, and fluorescence-activated cell sorting of bovine lymphocytes. These findings were presented at the International Veterinary Immunology Symposium in Australia (http://www.ivis2016.org/). Anti-cytokine monoclonal antibodies are useful in measuring cellular immune correlates of vaccination in livestock.