Most immunoassays require the addition of protein, detergent, or polymer to decrease non-specific binding. Although not common, it is possible that these substances can influence antigen-antibody binding. Another consideration is the requirement for some level of fetal bovine serum in the hybridoma screening assay. If your antigen is found in serum, or affected by serum components, it can be more difficult to obtain useful antibodies.
If you know or suspect that serum components bind or modify your antigen, or if serum contains homologous antigen, we will need to discuss alternative approaches before starting your project. Please contact us to discuss your project.