Cell Culture Services

How will you ship my cells?

Frozen and shipped on dry ice. We HIGHLY RECOMMEND thawing and culturing a vial immediately upon receipt.

How should I produce the antibody?

There are a variety of different flasks, vessels, and bioreactors for growing hybridoma cells as well as different media and additives. We recommend gradually weaning the cells from the media we grew them in before freezing. We are happy to send you small amounts of our media to help with this.

Two cautions:

    • All hybridoma cells lines have an inherent instability that leads to cells that grow but do not express antibody or express antibody at low levels. These so-called “non-producers” will tend to overpopulate the culture as time goes on. The solution is to routinely sublcone the line and to freeze cells at different stages of the process; for example, before the line is subcloned, after subclones have been selected and expanded, before seeding a bioreactor, after culture in a bioreactor, etc. Having these frozen stocks make it easier to go back and determine where in the process the problem began if you see loss of antibody production.

 

  • Always archive the line by freezing multiple vials, at different times, and checking to insure that the cells will thaw successfully and produce the expected amount of antibody. It is also critically important, along with having multiple batches of frozen cells, to have them stored in multiple liquid nitrogen freezers. We can’t stress this enough: store cells in multiple freezers!

And of course, we are here to help produce the antibody so that you don’t have to do this.

What kind of antibody yields can I expect?

Production levels typically run from 5-200 μg/ml in spent culture media.

Can we produce the monoclonal antibody in ascites?

You can, but our recommendation is to grow it in culture. We believe it is more humane and there are no longer overriding technical reasons to make ascites. Also, antibodies purified from ascites typically contain varying amounts of normal mouse immunoglobulin.

 

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