Growing Hybridomas

What are your methods?

Our standard media for growing established hybridoma cells is DMEM containing 8-10% fetal bovine serum supplemented with glutamine (2 mM final concentration) and 50 U/ml penicillin/streptomycin.  Cell densities are between 2 x 105 to 1 x 106 cells/ml in 8% CO2 at 37⁰.  Doubling times are approximately 20-22 hrs.

Will this serum work for my project?

Please contact us if you need help growing your cells.  Not all fetal bovine serums are identical and we can send you small amounts of our media to help you get your cultures going.  We can also share with you our approach to growing cells under serum-free conditions.

 

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