Production & Purification

How is the antibody produced?

By in vitro production: cells are cultured in roller bottles or flasks in serum free media or the media of your choice. Antibody is purified on protein G with low pH elution into Tris neutralizing buffer. We analyze the purified antibody using SDS-PAGE and dynamic light scattering.

How will I receive the antibody?

Purified antibody will be provided to you in a buffer of your choice. If you do not specify a preferred storage buffer, we recommend storing antibody in 50% glycerol/water at -20°C or in phosphate-buffered saline containing 0.02% sodium azide.

What information will I receive with the purified antibody?

A product data sheet is supplied outlining the process used for purification, estimated percent homogeneity, concentration, buffer, recommended storage conditions, and isotype.

Who owns the cell lines at the end of the project?

Once payment is received, you do.

How long does it take to produce and purify the antibody?

Starting with frozen cells, this process takes approximately 4-6 weeks, depending on the number of cell lines selected for production and the growth characteristics of the cell lines.

How is pricing determined for this stage?

Pricing for this stage is set in our hybridoma development package. Price may vary if an extra number of hybridomas are selected for antibody production or if larger amounts of antibody are required.

 

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