Case Study #2

Reagent Antibodies for Evaluation of Drug Levels in Patient Sera

Antibodies Specific for Parent Small Molecule Drug Over Potential Metabolites

Mice were immunospressed with the metabolite (oxide form of the drug) and then challenged with the parent drug.  Following fusion, clones producing specific antibody to the parent drug were selected over clones producing antibodies to the metabolite.  An ELISA was then developed using the parent drug specific antibody to detect drug in patient sera samples.