GMA-012

Background

Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, factor VIII circulates in an inactive form bound to von Willebrand factor. Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca²⁺. Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA-012 (also known as R8B12) recognizes the discontinuous epitope of residues 497-510 and 584-593 in the A2 domain of FVIII,¹ and is suitable for purification of FVIII,² ELISA, sandwich ELISA³, Western blotting, and bio-layer interferometry applications.