Clone GMA-8002
Background
Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, FVIII circulates in an inactive form bound to von Willebrand factor. Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca2+. Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA-8002 (also known as 2-116)1 recognizes the A1 domain of FVIII, and is suitable for bio-layer interferometry pairing and sandwich ELISA applications.
More Information
Description
- Species Reactivity:Human, Canine
- Source:Murine
- Specificity:A1 domain
- Immunogen:B-domain deleted recombinant Human Factor VIII
- Isotype:IgG2a
Application
- ELISA:Binds the A1 domain of recombinant human factor VIII in solid-phase ELISA.
- Immunoblotting:Not recommended.
- Inhibition:Does not inhibit in aPTT clotting assay.
Data
Antibody References
- R.J. Summers, S.L. Meeks, J.F. Healey, H.C. Brown, E.T. Parker, C.L. Kempton, C.B. Doering, P.Lollar. (2011). Blood. 117(11) :3190-3198.
- H. Wakabayashi, A.E. Griffiths, P.J.Fay. (2010). J. Biol. Chem. of Biological Chemistry 285(33):25176–2518.
- J.A. Dumont, T. Liu, S.C. Low, X.Zhang, G. Kamphaus, P. Sakorafas, C. Fraley, D. Drager, T. Reidy, J. McCue, H.W.G.Franck, E.P. Merricks, T.C. Nichols, A. J. Bitoni, G. F. Pierce, H. Jiang. (2012). Blood. 119(13):3024-3030.
- S. Krishnamoorthy, T. Liu, D. Drager, S. Patarroyo-White, E.S. Chhabra, R. Peters, N. Josephson, D. Lillicrap, R.S. Blumberg, G. F. Pierce, H. Hiang. (2016). Cell Immunol. 301:30-39.
- P. Lapalud, T.Ali, C. Cayzac, E. Mathieu-Dupas, H. Levesque, C. Pfeiffer, J. Balicchi, Y. Gruel, J.Y.Borg, J.F. Schved, C. Granier, G. Lavigne-Lissalde. (2012). J. Thromb. Haemost. 10(9): 1814–1822.
- P. Lapalud, C. Rothschild, E. Mathieu-Dupas, J. Balicchi, Y. Gruel, D. Laune, F. Molina,*J. F. Schved, C. Granier, G. Lavigne-Lissalde (2015). J. Thromb. Haemost, 13: 540–54.
$240.00 – $600.00