Many variables determine the specificity, affinity, isotype, and number of distinct monoclonal antibodies you obtain following somatic cell fusion of antibody-producing splenocytes and myeloma cells. Investigators need high-affinity antibodies and oftentimes large numbers of distinct specificities, so immunization protocols tend to run weeks or months. These longer protocols typically require up to a milligram of antigen to immunize several animals multiple times over several months. Fusion only occurs when there is a high titer serum immune response.
As an alternative to our standard immunization protocol, the Repetitive Immunizations Multiple Sites (RIMMS) strategy uses lymphocytes from regional draining lymph nodes and a significantly shorter immunization schedule: two weeks instead of several months. Along with using less antigen, this approach allows the investigator to obtain hybridomas in a month.RIMMS is an ideal strategy when you need one or several monoclonal antibodies quickly. The standard immunization protocol is recommended when you need large numbers of distinct monoclonal antibodies.
- Requires less antigen for immunization
- Can produce positive hybridoma antibodies in 4 weeks
- Useful when an antibody is needed quickly
- Rat Monoclonal Antibodies: Custom Development
- Hybridoma Screening Strategies
- Variable Region Sequencing
- mAb-Antigen Affinity Using Octet
- Large Scale Antibody Production and Purification
1. Caterson, B. et al. (1983) J. Biol. Chem. 258, 8848-8854.
2. Kilpatrick, K.E., et al. (1997) Hybridoma 16, 381-389