GMA-8023

Murine Anti- Factor VIII

Clone GMA-8023

Background

Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, FVIII circulates in an inactive form bound to von Willebrand factor. Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca2+. Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA-8023 (also known as 2G10) recognizes the A2 domain of FVIII1 and can be used in conjunction with GMA-8024 as a sandwich ELISA pair for detection of human FVIII. It does not bind murine, rhesus or cynomolgus FVIII.

More Information:

Product Datasheet, pdf

Description

Species Reactivity: Human
Source: Murine
Specificity: A2 domain
Immunogen: B-domain deleted recombinant Human Factor VIII
Isotype: IgG2a

Application

ELISA: Binds the A2 domain of recombinant human factor VIII in solid-phase ELISA.
Immunoblotting: Not recommended.
BLI: kdis = 2 x 10-1 sec-1 by biolayer interferometry.

Data:

Purchase GMA-8023

  • 0.5 mg/$660.00
  • 0.1 mg/$265.00
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Antibody References:

  1. R.C. Markovitz et al. (2013). Blood. 121(14):2785-2795.
  2. H. Chen et al. (2019). Scientific Reports. 9:16838.
  3. L. Zhang et al. (2020). Molecular Therapy: Methods & Clinical Development. 17:13-20.
  4. H. Chun et al. (2020). J Thromb Haemost. 19(4):954-966.