GMAb’s optimized antibody sequencing service provides highly accurate hybridoma cell line sequencing with the fastest turnaround time on the market. Our optimized approach, processed in our newly expanded laboratory, makes sequences available in an average of 3 business days.
Sequence your antibody to safeguard from the possibility of losing the hybridoma cell line. This critical piece of information is required for patent applications. Once your sequence is identified, we can express the sequence recombinantly to validate binding and create the ideal format for your application. Our comprehensive suite of recombinant antibody production services ensures that you have the exact tools you need for your research or therapeutic goals. Also consider safeguarding your cell lines, with dual location storage service at GMAb.
Recombinant Services Include:
Chimeric Antibody Production Services: Tailor antibodies by combining variable regions from one species with constant regions from another, optimizing performance and compatibility.
Fab/scFv Production Services: Generate fragment antibodies, such as Fab and scFv, designed for specific applications requiring smaller size and enhanced tissue penetration.
When you choose our antibody sequencing service, you receive:
Full Sequence of VH and VL Variable Domain Regions: Obtain comprehensive sequences for both heavy and light chains, essential for understanding your antibody structure.
Supporting Data for Sequence Correctness: Rest assured with detailed data that confirms the accuracy of the sequence, ensuring reliability and precision.
100% Coverage with Excellent Accuracy: Benefit from guaranteed full coverage of the desired regions, paired with outstanding accuracy for dependable results.
This thorough approach ensures that you have all the necessary information not only for current applications but also for future developments.
Our Approach
All antibodies have the same basic protein structure but exhibit sequence variability in the regions that contact the antigen. Our variable domain sequencing identifies these VH and VL sequences in the heavy and light chain of the immunoglobulins from the mRNA encoding the antibody and expressed in the hybridoma cell line.
Types of Antibodies and Species
Our sequencing services cater to a wide array of antibody types, including:
IgG, IgA, IgM
We also provide sequencing for antibodies from a variety of species such as human, mouse, rat, rabbit, hamster, sheep, and goat, ensuring comprehensive coverage across diverse research needs.
The antibody sequencing services at Green Mountain Antibodies can be completed in as fast as 3 days from the receipt of your cell pellet.
General Workflow for Antibody Sequencing
1. Sample Preparation: Begin by preparing your cell pellet for analysis, ensuring it meets the necessary quality standards for optimal results.
2. Sequencing Techniques: Utilize advanced sequencing techniques to accurately determine the amino acid sequence from the antibody cDNA. This step is critical for ensuring precision and reliability in the results.
3. Data Analysis: Analyze the sequencing data to interpret and verify the results. This involves comparing the sequences to known databases and identifying unique characteristics.
4. Final Reporting: Compile the findings into a comprehensive report that details the entire process, antibody sequence results, and any recommendations or additional observations. Our sample report provides a preview of what you can expect.
By following this thorough workflow, you can be confident in obtaining accurate and reliable antibody sequences.
Why Green Mountain Antibodies?
Capture the entire variable domain: By utilizing rapid amplification of cDNA ends (RACE), we capture the entire variable domain plus the leader sequence and 5’ UTR, ensuring that we report every residue in the variable domain that makes YOUR antibody unique.
Optimized sequencing approach: Nanopore sequencing combines the best features of other sequencing technologies – the many reads of NGS (allowing us to see the complexity of the hybridoma) with the speed of Sanger sequencing (overnight results). Plus, length is no longer a limiting factor, so we can easily cover as much of the sequence as needed. Improvements in ONT has made the sequence highly accurate (>99.99% with >15 reads). We have extensively compared with other approaches and see 100% sequence agreement.
We know more: Every sequence is analyzed by an experienced molecular biologist to verify functional antibody sequences and ensure no process contamination. Sequence your entire project with us to get in depth phylogenetic comparison and summary of sequence liabilities. If your hybridoma is identified as complex, we can express your sequences recombinantly, assay them here, and tell you exactly which heavy and light chains affect binding (plus some insights on developability).
Data analysis involves bioinformatics tools that align and interpret sequencing reads to reconstruct the antibody’s genetic sequence, allowing for insights into its structure and function.
Speed: Biotechnology is a fast-paced field, and your time is important. That’s why our antibody sequencing processes are built for speed, while still providing the highest level of accuracy. Our average turnaround time for standard VH/VL sequencing is 3 business days from pellet to report.
Quality: Quality control measures include assessing the integrity and purity of the sample, ensuring accurate sequencing through calibration of equipment, and verifying data through replicates and controls.
Service Details
We accept cell pellets or frozen vials of hybridoma cells or clonal B cells. Ask us for detailed instructions on alternative methods to submit your sample. GMAb can also culture your cells for the sequencing process, so you can avoid sample preparation time at your facility.
What You Need to Provide
Purity: A monoclonal cell line ensures ease of sequencing. If multiple lines are included in your sample, please let us know for appropriate planning.
Sample Types: Acceptable samples include cell pellets or frozen vials of cells. If you need guidance on other submission methods, feel free to reach out.
By meeting these criteria, you can facilitate a smooth and efficient sequencing process. Remember, we’re here to help with culturing and preparation to streamline your workflow.
Our experts can design schemes for all your sequencing needs, including antibodies from any species (mouse, rat, human, rabbit, hamster, camel, sheep, etc.) and any constant region (IgG1, IgG2a, IgG2b, IgG3, IgG4, IgM, IgE, IgA, IgK, IgL, etc.) Let us know what you need and we’ll design a solution.
If you find your samples include multiple sequences, we can assist with subcloning to monoclonality, or expressing the desired sequence recombinantly.
Applications
- Hybridoma identification
- Antibody engineering
- Expression in alternate platforms
- Antibody humanization
- Confirm diversity
- Antibody binning
- Antibody immortalization and preservation
Sequencing Request Form
For multiple cell lines fill in the form and send to Sales@greenmoab.com for a quote.
Download Form
Explore More Services
- Antibody Development Services
- ELISA Assay Development
- Octet Analysis
- Gator Analysis
- Antibody Characterization, including purity and stability testing
- Large Scale Antibody Production and Purification, including custom fluorescent dye or biotin conjugations
- Recombinant Antibody Expression
- Cell line archival and dual site storage
Custom Monoclonal Antibody Production Services
When it comes to custom monoclonal antibody (MAb) production services, a range of specialized options are available to meet diverse research and development needs:
1. Mouse Monoclonal Antibody Production
Benefit from time-tested methods for creating monoclonal antibodies using mouse models, renowned for their reliability and efficacy in various applications.
2. Support for WB/IHC Antibody Production
Secure antibodies that are guaranteed to work effectively in Western Blot (WB) and Immunohistochemistry (IHC) applications, ensuring your experiments yield reliable results.
3. Antibody Pairs Screening Service
Speed up assay development by screening and identifying optimal pairs of monoclonal antibodies for your diagnostic or research needs.
4. Custom Conjugation Services
Custom antibody conjugation services for fluorescent dyes and biotin offer various specialized solutions to modify antibodies for specific research needs. These services tailor antibodies by attaching different molecules to enhance their functionality in applications like diagnostics, therapeutics, and research; ensuring maximum sensitivity and specificity.