Antibody Sequencing

NEW Hybridoma Antibody Variable Domain Sequencing Service

Sequences are available in as little as 7 days

GMAb responded to customer needs to provide sequencing results faster and sooner in the process. Our optimized approach, processed in our newly expanded laboratory, makes sequences available in as few as 7 days with higher accuracy.

All IgG antibodies have the same basic protein structure but exhibit sequence variability in the regions that contact the antigen. Antibody sequencing identifies these regions, known as the “variable domain”. The variable domain encodes approximately 100 amino acids at the amino-terminal of both the light and heavy chains of immunoglobulins. By utilizing 5’ rapid amplification of cDNA ends (RACE), we capture the entire variable domain, ensuring that we report every residue in the variable domain that makes YOUR antibody unique.

Sequence your antibody to safeguard from the possibility of losing the hybridoma cell line. This critical piece of information is also required for patent applications. We accept cell pellets or frozen vials of hybridoma cells. GMAb will culture your cells for the sequencing process, so you can avoid sample preparation time at your facility. The antibody sequencing services at Green Mountain Antibodies can be completed in as fast as 7 days. See our sample report.

Monoclonal antibodies to the same antigen target can have different variable domain antibody sequences recognizing different epitopes. This can be particularly important in creating a library of clones. Knowing that antibodies recognize different parts of the target is important for binning and confirming diversification, especially in lead generation hybridoma development projects. Antibody sequencing of the variable domains is often the first step in antibody engineering to immortalize the selected epitope.

Hybridoma cell lines synthesize antibodies with identical antigen specificity and are an ideal source of genetic material for the preparation of recombinant molecules. Employing GMAb’s antibody sequencing services allows you to recreate your valuable antibody via recombinant expression methods.

Uses

Need custom sequencing?  We can also sequence full-length antibodies and non-mouse antibodies. Let us know what you need and we’ll find a solution.

Want to find out more about your antibody? We can provide additional annotation, including the leader sequence and predicted V, D, and J gene identity.