GMA-8009

Background

Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, FVIII circulates in an inactive form bound to von Willebrand factor. Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca²⁺. Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA-8009 (also known as 1D4 or 1D4.1B)¹ recognizes the A2 domain of FVIII and inhibits FVIII activation by thrombin or factor Xa. It is suitable for immunohistochemistry and FACS², ELISA and bio-layer interferometry pairing experiments.