GMA-8014

Murine Anti- Factor VIII

Clone GMA-8014

Background

Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, FVIII circulates in an inactive form bound to von Willebrand factor (vWF). Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca2+. Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA-8014 (also known as 3G6 or 3G6.1B)1 recognizes the C2 domain of FVIII. The antibody strongly inhibits FVIII activation and is unaffected by the presence of vWF or phospholipid membranes.1 GMA-8014 detects FVIII C2 domain by surface plasmon resonance,2,3 and is suitable for ELISA and bio-layer interferometry pairing experiments.

More Information:

Product Datasheet, pdf

Description

Species Reactivity: Human
Source: Murine
Specificity: C2 Domain
Immunogen: B-domain deleted recombinant Human Factor VIII
Isotype: IgG2b

Application

ELISA: Binds the C2 domain of recombinant human factor VIII in solid-phase ELISA..
Immunoblotting: Does not blot.
Inhibition: Strongly inhibitory in aPTT clotting assay.1

Data:

Purchase GMA-8014

  • 0.5 mg/$660.00
  • 0.1 mg/$265.00
For Larger Amounts Please Call:
(802) 865-6230
Quantity:
Continue Shopping

Antibody References:

  1. S.L. Meeks et al. (2007). Blood. 110(13):4234-4242.
  2. P.T. Nguyen et al. (2014). Blood. 123(17):2732-2739.
  3. N.C. Leksa et al. (2017). J Thromb Haemost. 15(6):1167-1179.
  4. G.S. Pandey et al. (2013). Nat Med. 19(10):1318–1324.