Murine Anti-Factor IX
Factor IX (FIX) is a vitamin K-dependent zymogen that plays an essential role in the coagulation cascade leading to thrombus formation. In the presence of calcium, activated Factor IX (FIXa) complexes with Factor VIIIa on phospholipid surfaces to create the tenase complex, which converts Factor X to its activated form. Absent or defective FIX is the cause of the X-linked recessive bleeding disorder hemophilia B. GMA-001 (BC2) binds to the N-terminal Gla domain of FIX and the light chain of FIXa, as detected by Western blot and ELISA. In addition, GMA-001 prolongs clotting time in APTT-based plasma clotting assays, and prevents arterial thrombosis in vivo in a rat model1.
- Species Reactivity:Human, Rat
- Specificity:Gla domain (residues 1-40) of FIX/FIXa
- Immunogen:Human Factor IX
- ELISA:Binds immobilized Human and Rat FIX, Human FIXa only in the presence of calcium.
- Immunoblotting:Western blot detects Human FIX and light chain of Human FIXa under reduced conditions in presence of calcium.
- Inhibition:Prolongs plasma clot time in APTT clotting assay.
- G.Z. Feuerstein et al. (1999). Thromb. Haemost. 82(5):1443-5.
- G.Z. Feuerstein et al. (1999). Arterioscler Thromb Vasc Biol. 19(10):2554-62.
- J. Tie et al. (2011). Blood. 117(10):2967-2974.
- J. A. Haque et al. (2014). Blood. 123(4):582-589.
- M. A. Chiasson et al. (2020). eLife. 9:1-25.
- W. Gao et al. (2020). Blood Advances. (4)15:3659-3667.
$265.00 – $660.00