GMA-8016

Murine Anti- Factor VIII

Clone GMA-8016

Background

Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, FVIII circulates in an inactive form bound to von Willebrand factor. Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca2+ . Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA- 8016 (also known as 4C7 or 4C7.1B) 1 recognizes the A2 domain of FVIII, and is suitable for immunopurification,2 ELISA, and bio-layer interferometry pairing experiments.

More Information:

Product Datasheet, pdf

Description

Species Reactivity: Human
Source: Murine
Specificity: A2 domain
Immunogen: B-domain deleted recombinant Human Factor VIII
Isotype: IgG2a

Application

ELISA: Binds immobilized human FVIII.
Immunoblotting: Not recommended.
Inhibition: Not inhibitory in aPTT clotting assay.1

Data:

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Antibody References:

  1. R.C. Markovitz et al. (2013). Blood. 121(14):2785-2795.
  2. A. van der Flier et al. (2015). PLoS One. 10(4): e0124930.
  3. S. Krishnamoorthy et al. (2016). Cell Immunol. 301:30-39.
  4. J.A. Dumont et al. (2012). Blood. 119(13):3024-3030.
  5. Y. Buyue et al. (2014). PLoS ONE. 9(11):e113600.
  6. J. Firrman et al. (2020). Molecular Therapy: Methods & Clinical Development. 17:328-336.
  7. L. L. Swystun et al. (2019). J Thromb Haemost.. 17(4): 681–694.