GMA-8002

Murine Anti- Factor VIII

Clone GMA-8002

Background

Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, FVIII circulates in an inactive form bound to von Willebrand factor. Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca2+. Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA-8002 (also known as 2-116)1 recognizes the A1 domain of FVIII, and is suitable for bio-layer interferometry pairing and sandwich ELISA applications.

More Information:

Product Datasheet, pdf

Description

Species Reactivity: Human, Canine2
Source: Murine
Specificity: A1 domain
Immunogen: B-domain deleted recombinant Human Factor VIII
Isotype: IgG2a

Application

ELISA: Binds the A1 domain of recombinant human factor VIII in solid-phase ELISA.
Immunoblotting: Not recommended.
Inhibition: Does not inhibit in aPTT clotting assay.

Data:

Purchase GMA-8002

  • 0.5 mg/$660.00
  • 0.1 mg/$265.00
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Antibody References:

  1. R.J. Summers et al. (2011). Blood. 117(11):3190-3198.
  2. J.A. Dumont et al. (2012). Blood. 119(13):3024-3030.
  3. H. Wakabayashi et al. (2010). J. Biol. Chem. of Biological Chemistry. 285(33):25176–2518.
  4. S. Krishnamoorthy et al. (2016). Cell Immunol. 301:30-39.
  5. P. Lapalud et al. (2012). J. Thromb. Haemost. 10(9):1814–1822.
  6. P. Lapalud et al. (2015). J. Thromb. Haemost. 13:540–547.
  7. M. Elnaggar et al. (2020). Molecular Therapy: Methods & Clinical Development. 17:1-12.
  8. L. L. Swystun et al. (2019). J Thromb Haemost.. 17(4): 681–694.