GMA-8003

Murine Anti- Factor VIII

Clone GMA-8003

Background

Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, FVIII circulates in an inactive form bound to von Willebrand factor. Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca2+. Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA-8003 (also known as 2-117)1 recognizes the C2 domain of FVIII and is suitable for ELISA, bio-layer interferometry pairing, and surface plasmon resonance studies.2

More Information:

Product Datasheet, pdf

Description

Species Reactivity: Human
Source: Murine
Specificity: C2 Domain
Immunogen: B-domain deleted recombinant Human Factor VIII
Isotype: IgG2a

Application

ELISA: Binds the C2 domain of recombinant human factor VIII in solid-phase ELISA.
Immunoblotting: Not recommended.
Inhibition: Not inhibitory in aPTT clotting assay.1

Data:

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  • 0.5 mg/$660.00
  • 0.1 mg/$265.00
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Antibody References:

  1. S. Meeks et al. (2007). Blood. 110(13):4234-4242.
  2. P.T. Nguyen et al. (2014). Blood. 123(17):2732-2739.
  3. R. Summers et al. (2011). Blood. 117(11):3190-8.
  4. G.S. Pandey et al. (2013). Nat Med. 19(10):1318–1324.
  5. H. Wakabayashi et al. (2014). The Journal of Biological Chemistry. 289(20):14020–14029.
  6. H. Wakabayashi, P.J. Fay. (2013). The Journal of Biological Chemistry. 288(43):31289–31297.
  7. H. Wakabayashi, P.J. Fay. (2013). Biochemistry. 52(22):3921–3929.
  8. H. Wakabayashi, P.J. Fay. (2013). Biochem J. 452(2):293–301.
  9. M. Takeyama et al. (2013). Thromb Haemost. 109(2):187–198.
  10. H. Wakabayashi et al. (2011). The Journal of Biological Chemistry. 286(29):25748 –25755.