Antibody Development Services
The starting point is a detailed discussion of your needs and goals so we best understand which antibody services will be right for you. We will ask questions about the chemistry and biology of your antigen target. This information often reveals barriers or problems that require different or alternative approaches. The project plan then evolves; setting expectations, describing timelines, explaining cost options, and discussing possible solutions to anticipated problems. This is where our experience with similar targets comes in to play.Green Mountain Antibodies can support you in developing a monoclonal and polyclonal antibody against your target. Both programs can be managed side by side, allowing your reagents to be used concurrently. The quantity of antibody required, and final application will allow us to guide whether a mouse monoclonal or rabbit polyclonal antibody will be best for you. For anti-idiotypic programs, the goal is often to develop both a monoclonal antibody for PK assays and a polyclonal antibody for ADA assays. If you do not have purified antigen, we can offer suggestions for obtaining recombinant protein or help with peptide selection, synthesis and conjugation. We’ll offer our opinions on the best way forward. Our knowledge of protein chemistry, immunochemistry, and assay development supports decision making throughout the process. You might have a constrained budget or other limitations. You may have knowledge of previous antibody development efforts or information in the literature suggesting helpful approaches. Our way of working is to learn from your previous experiences and share what we know. We believe working as your antibody services partner offers the greatest chance of success.
Monoclonal Antibody Production and Purification Services
Our hybridoma development process includes multiple rounds of subcloning to assure your line is monoclonal and stable. The process allows us to select clones with higher antibody production levels.After investing time and resources making a cell line that produces a monoclonal antibody, you will realize substantial savings (cost/mg) over purchasing the antibody from a commercial source. This also removes concerns of continuity of supply and inventory availability. The best antibody for your application will be available when you need it. GMAb produces antibody via fixed volume tissue culture vessels or bench-top bioreactors into the gram scale. Monoclonal antibody is then purified by Protein G and extensively characterized. Chemical labeling of the purified antibody with biotin, fluorescent dyes or proteins is available.
Antibody Characterization Services
Green Mountain Antibodies can characterize the antibody to assure optimal selection and performance, as well as confirm lot to lot reproducibility of your selected antibody. Characterization services include:
Mouse Monoclonal Antibodies: Hybridoma Development
Learn about the advantages of the Green Mountain Antibodies experienceMouse Monoclonal Antibodies: Hybridoma Development
Polyclonal Antibody Production
A perfect complement to your monoclonal antibody developmentPolyclonal Antibody Production
We support our customers through discovery, pre-clinical and clinical trialsAnti-Idiotypic Antibodies
Repetitive Immunizations Multiple Sites (RIMMS)
Learn more about the rapid immunization alternativeRepetitive Immunizations Multiple Sites (RIMMS)
MAB PRODUCTION & PURIFICATION
Large Scale Antibody Production and Purification
Benefit from our cell culture expertise to scale up to desired quantitiesLarge Scale Antibody Production and Purification
Considerations when sending external cell lines
Documentation from an independent lab certifying that cells are mycoplasma freeMycoplasma Policy
ELISA Assay Development
Utilize our expertise to get the most specificity and sensitivity for your assayELISA Assay Development
Determine the physicochemical and immunochemical properties, biological activity, purity, impurities and quantity of a monoclonal antibodyAntibody Characterization
Thorough analysis of the kinetics between molecular interactionsOctet Analysis
Learn how we provide the correct and unique sequence in the hybridoma processAntibody Sequencing
Not sure what you need?
Our expert team will guide you every step of the way.