Green Mountain Antibodies internally developed a mouse monoclonal antibody against bovine serum albumin. The BSA antibody, GMA-630, binds BSA and BSA-conjugated peptides in solid phase ELISA. The monoclonal does not work in immunoblotting. The bovine serum albumin antibody is an IgG2b isotype and tests >98& pure by SDS-PAGE.
BSA (bovine serum albumin), also known as Fraction V, is a soluble 66.5 kDa protein derived from cows. Albumin makes up about half of the serum blood proteins and is often used as a protein concentration standard, stabilizer for enzymes or blocking agent in laboratory settings. BSA is often used as a conjugate for small molecules, such as peptides, to increase their size and confer properties more amenable for assays such as solid phase ELISAs.
In experiments where BSA-conjugates are used, rigorous experiments should include negative controls that demonstrate that responses are to the peptide of interest, and not the BSA portion of the molecule- the anti-BSA antibody can be used as part of this control set.
In a recent Octet experiment to determine affinity between a custom antibody and its antigen, signals were too low to be accurately interpreted, possibly due to steric issues around presentation of the peptide. To overcome this, a method was developed in which GMA-630 was used to capture a Bovine Serum Albumin-conjugated peptide and thus present the peptide in an orientation to be better bound by its antibody, increasing signal to measurable levels.
Applications
ELISA: Binds BSA and peptide-BSA in solid-phase ELISA.
Immunoblotting: Does not blot.
Antibody to BSA Formulation:
Lyophilized from a 1 mg/ml solution with 10 mM NaH2PO4 0.15 M NaCl, 1.0% (w/v) mannitol, pH 7.4. Concentration determined by absorbance measurement at 280 nm and using an extinction coefficient of 1.4 (E0.1%).
Storage: Aliquot and store at -20° C for prolonged periods. Avoid freeze/thaw cycles. Alternatively, bring to 0.01% (w/v) sodium azide and store at 4° C.
Antibody to BSA is produced via in vitro production methods, under serum free conditions, and purified by Protein G affinity chromatography. Custom conjugation is available. Products typically ship within 24 hours after receiving the purchase order.
All Products
All Products![](https://greenmoab.com/wp-content/uploads/2023/01/vials-3.png)
Human Coagulation Factors and Plasma Antigens
All Products![](https://greenmoab.com/wp-content/uploads/2023/01/vials-1.png)
Mouse Coagulation Factors and Plasma Antigens
All Products![](https://greenmoab.com/wp-content/uploads/2023/01/vials.png)
Control Antigens
All Products![](https://greenmoab.com/wp-content/uploads/2019/01/antibody-100px.png)
![NEAH Scientists](https://greenmoab.com/wp-content/uploads/2019/01/NEAH-Scientists-2.jpg)
Supporting Livestock Vaccine Development to Advance Food Security Worldwide
NEAH is a vital laboratory source of the immunological tools—monoclonal and recombinant antibodies—that are the building blocks for livestock infectious disease vaccine research and development (particularly goats and sheep). To help enable our vision, NEAH and Pentland Immunologics have identified and established manufacturing service agreements with key companies including Green Mountain Antibodies. These facilities have a proven track record in the supply of curated immunological reagents for the research community. Purchase NEAH antibodies through their website here.