Bovine Serum Albumin Antibody (BSA)

All Products

Green Mountain Antibodies internally developed a mouse monoclonal antibody against bovine serum albumin. The BSA antibody, GMA-630, binds BSA and BSA-conjugated peptides in solid phase ELISA. The monoclonal does not work in immunoblotting. The bovine serum albumin antibody is an IgG2b isotype and tests >98& pure by SDS-PAGE.

BSA (bovine serum albumin), also known as Fraction V, is a soluble 66.5 kDa protein derived from cows. Albumin makes up about half of the serum blood proteins and is often used as a protein concentration standard, stabilizer for enzymes or blocking agent in laboratory settings. BSA is often used as a conjugate for small molecules, such as peptides, to increase their size and confer properties more amenable for assays such as solid phase ELISAs.

In experiments where BSA-conjugates are used, rigorous experiments should include negative controls that demonstrate that responses are to the peptide of interest, and not the BSA portion of the molecule- the anti-BSA antibody can be used as part of this control set.

In a recent Octet experiment to determine affinity between a custom antibody and its antigen, signals were too low to be accurately interpreted, possibly due to steric issues around presentation of the peptide. To overcome this, a method was developed in which GMA-630 was used to capture a Bovine Serum Albumin-conjugated peptide and thus present the peptide in an orientation to be better bound by its antibody, increasing signal to measurable levels.

Applications

ELISA: Binds BSA and peptide-BSA in solid-phase ELISA.

Immunoblotting: Does not blot.

Antibody to BSA Formulation:
Lyophilized from a 1 mg/ml solution with 10 mM NaH2PO4 0.15 M NaCl, 1.0% (w/v) mannitol, pH 7.4. Concentration determined by absorbance measurement at 280 nm and using an extinction coefficient of 1.4 (E0.1%).

Storage: Aliquot and store at -20° C for prolonged periods. Avoid freeze/thaw cycles. Alternatively, bring to 0.01% (w/v) sodium azide and store at 4° C.

Antibody to BSA is produced via in vitro production methods, under serum free conditions, and purified by Protein G affinity chromatography. Custom conjugation is available. Products typically ship within 24 hours after receiving the purchase order.

All Products

All Products
Catalog #
Specificity
Antigen
Applications
GMA-630
BSA and peptide-BSA conjugates.
Bovine Serum Albumin Antibody
ELISA
Human Coagulation Factors and Plasma Antigens

Human Coagulation Factors and Plasma Antigens

All Products
Catalog #
Specificity
Antigen
Applications
GMA-001
Gla domain (residues 1-40) of FIX/FIXa
Factor IX
calcium dependence, ELISA, western blot, inhibitory
GMA-004
Factor XIII
ELISA
GMA-012
A2 domain
Factor VIII
ELISA, western blot, biolayer interferometry, immunopurification
GMA-013
Kringles 1-3 segment of plasminogen
Plasminogen / Angiostatin
ELISA, western blot
GMA-014
α-2-macroglobulin
Alpha-2-Macroglobulin
ELISA, western blot
GMA-016
Kringles 1-4 segment of plasminogen
Plasminogen / Angiostatin
ELISA, western blot
GMA-020
a-thrombin B-chain (residues 321- 579 of prothrombin)
Thrombin
ELISA, western blot
GMA-022
vWF
ELISA, western blot
GMA-030
Kringle 5-B chain
Plasminogen / Angiostatin
ELISA
GMA-033
B subunit
Factor XIII
ELISA, western blot
GMA-034
Human fibrinogen
Fibrinogen
ELISA, western blot
GMA-035
Human fibrinogen
Fibrinogen
ELISA, western blot
GMA-036
C4b Binding Protein
ELISA, immunopurification
GMA-039
Kringle 5-B chain
Plasminogen / Angiostatin
ELISA, western blot, biolayer interferometry, immunopurification
GMA-043
Tissue Plasminogen Activator
ELISA, western blot
GMA-044
FV, FVa heavy chain
Factor V
ELISA, western blot
GMA-046
Protein S
ELISA
GMA-047
Protein S
ELISA, western blot
GMA-053
Protein C
Protein C
ELISA, western blot
GMA-054
activation region of Protein C
Protein C
ELISA, western blot, calcium dependence
GMA-067
Protein C heavy chain
Protein C
ELISA, western blot
GMA-070
Factor XI/XIa
Factor XI
ELISA, western blot, immunopurification
GMA-072
Factor XI/XIa
Factor XI
ELISA, western blot
GMA-086
plasminogen
Plasminogen / Angiostatin
ELISA, western blot
GMA-091
prothrombin fragment 2
Prothrombin
ELISA, western blot
GMA-093
Protein C heavy chain
Protein C
ELISA, western blot
GMA-095
Prethrombin-1 (residues 272-579 of prothrombin)
Prothrombin
ELISA, western blot
GMA-097
prethrombin 1, prethrombin 2, prothrombin
Prothrombin
western blot
GMA-099
transferrin
Transferrin
ELISA, western blot
GMA-101
FIX/FIXa
Factor IX
immunohistochemistry, ELISA
GMA-102
Heavy chain of FIX/FIXa
Factor IX
ELISA, western blot, inhibitory, biolayer interferometry
GMA-164
Light chain of FIX/FIXa
Factor IX
ELISA, western blot,
GMA-121
Light chain of FIX/FIXa
Factor IX
ELISA, western blot
GMA-124
Light chain of FIX/FIXa
Factor IX
ELISA, western blot, biolayer interferometry
GMA-134 (previously known as GMA-133)
Human FIX (148T)
Factor IX
detects FIX with Thr 148 in ELISA, western blot, biolayer interferometry
GMA-138
FIX/IXa
Factor IX
ELISA, biolayer interferometry
GMA-142
Factor XII Heavy Chain
Factor XII
ELISA, western blot
GMA-145
Factor XII
Factor XII
western blot
GMA-140
Factor XII/XIIa
Factor XII
ELISA, western blot
GMA-144
Factor XII/XIIa
Factor XII
ELISA, western blot
GMA-148
Factor XII/XIIa
Factor XII
western blot
GMA-141
Factor XII/XIIa
Factor XII
ELISA, western blot
GMA-147
Factor XII/XIIa
Factor XII
western blot
GMA-146
Factor XII/XIIa
Factor XII
ELISA, western blot
GMA-143
Factor XII/XIIa
Factor XII
ELISA, western blot
GMA-162
Factor IXa heavy chain
Factor IX
ELISA, western blot
GMA-184
Light chain of FIXa
Factor IX
ELISA, western blot, biolayer interferometry
GMA-213
Factor VII/VIIa
Factor VII
ELISA, western blot, immunohistochemistry
GMA-219
Factor VII/VIIa
Factor VII
ELISA, western blot
GMA-320
Extracellular domain of tissue factor.
Tissue Factor
ELISA, western blot
GMA-340
Shedding cleavage site of GPIba
GPIba
ELISA, western blot
GMA-350
Disintegrin domain of ADAMTS13
ADAMTS13
ELISA, western blot, immunohistochemistry, immunopurification
GMA-505
Factor X/Xa
Factor X
ELISA, western blot
GMA-508
Factor X heavy chain
Factor X
ELISA, western blot
GMA-509
Factor X light chain
Factor X
ELISA, western blot
GMA-520
Factor X light chain
Factor X
ELISA, western blot
GMA-529
Factor X/Xa
Factor X
ELISA, western blot, inhibitory
GMA-550
Factor X/Xa
Factor X
ELISA, western blot
GMA-560
Factor X heavy chain
Factor X
calcium dependence, ELISA, western blot
GMA-800
Antithrombin
ELISA, inhibitory
GMA-900
Vitronectin
ELISA, western blot, immunopurification
GMA-5001
FV, FVa light chain
Factor V
ELISA, inhibitory, biolayer interferometry
GMA-5007
FV, FVa activation fragment
Factor V
ELISA, western blot, immunopurification
GMA-5017
FV/Va heavy chain, residues 307-5061
Factor V
ELISA, western blot
GMA-8001
A3 Domain
Factor VIII
ELISA, biolayer interferometry, inhibitory
GMA-8002
A1 domain
Factor VIII
ELISA, biolayer interferometry
GMA-8003
C2 Domain
Factor VIII
ELISA, biolayer interferometry, surface plasmon resonance
GMA-8004
A1 domain
Factor VIII
ELISA, biolayer interferometry, surface plasmon resonance
GMA-8005
A1 domain
Factor VIII
ELISA, biolayer interferometry
GMA-8006
C2 Domain
Factor VIII
ELISA, biolayer interferometry, flow cytometry, surface plasmon resonance, inhibitory
GMA-8008
C2 Domain
Factor VIII
ELISA, biolayer interferometry, surface plasmon resonance, inhibitory
GMA-8009
FVIII A2 domain (residues Glu604-Arg740)1
Factor VIII
ELISA, biolayer interferometry, immunohistochemistry, FACS, inhibitory
GMA-8010
Light Chain
Factor VIII
ELISA, biolayer interferometry, inhibitory
GMA-8011
C1 domain
Factor VIII
ELISA, surface plasmon resonance
GMA-8012
A1A3 domain
Factor VIII
ELISA, biolayer interferometry
GMA-8013
C2 Domain
Factor VIII
ELISA, biolayer interferometry, surface plasmon resonance, inhibitory
GMA-8014
C2 Domain
Factor VIII
ELISA, biolayer interferometry, surface plasmon resonance, inhibitory
GMA-8015
A2 domain
Factor VIII
ELISA, inhibitory
GMA-8016
A2 domain
Factor VIII
ELISA, biolayer interferometry, immunopurification
GMA-8017
A2 domain
Factor VIII
ELISA
GMA-8018
Light Chain
Factor VIII
ELISA, inhibitory
GMA-8019
Light Chain
Factor VIII
ELISA, inhibitory
GMA-8020
Light Chain
Factor VIII
ELISA, biolayer interferometry, inhibitory
GMA-8021
A2 domain
Factor VIII
ELISA, biolayer interferometry, inhibitory
GMA-8022
C2 Domain
Factor VIII
ELISA, inhibitory
GMA-8023
A2 domain
Factor VIII
ELISA, biolayer interferometry
GMA-8024
A2 domain
Factor VIII
ELISA
GMA-8025
C2 Domain
Factor VIII
ELISA, western blot
GMA-8026
C2 Domain
Factor VIII
ELISA, inhibitory
GMA-8027
C2 Domain
Factor VIII
ELISA
GMA-8028
A2 domain
Factor VIII
ELISA, western blot, inhibitory
GMA-8029
C2 Domain
Factor VIII
ELISA, surface plasmon resonance, inhibitory
GMA-8040
Light Chain
Factor VIII
ELISA, western blot
GMA-8041
Light Chain
Factor VIII
ELISA, western blot
GMA-8045
Light Chain
Factor VIII
ELISA
Bovine Immune Markers

Bovine Immune Markers

All Products
Catalog #
Specificity
Antigen
Applications
GMA-3011 (IL-A11)
Bovine CD41
CD4
-
GMA-3021 (IL-A21)
Bovine MHC Class II1
MHC Class II
-
GMA-3024 (IL-A24)
Bovine CD172a (myeloid cells)1
CD172a
-
GMA-3030 (IL-A30)
Bovine IgM1
IgM
ELISA
GMA-3060 (IL-A60)
Bovine IgG11
IgG1
ELISA
GMA-3071 (IL-A71)
Bovine IgA1
IgA
ELISA
GMA-3074
Bovine IgG2
IgG2
ELISA
GMA-3088 (IL-A88)
Bovine MHC Class I1
MHC Class I
-
GMA-3105 (IL-A105)
Bovine CD8α and CD8βα1
CD8
-
GMA-3111 (IL-A111)
Bovine CD251
CD25
-
GMA-3130 (IL-A130)
Bovine CD11b1
CD11b
-
Mouse Coagulation Factors and Plasma Antigens

Mouse Coagulation Factors and Plasma Antigens

All Products
Catalog #
Specificity
Antigen
Applications
GMA-737
Factor XIII
ELISA, western blot
GMA-739
Factor XIII
ELISA, western blot
GMA-740
Factor XIII
ELISA, western blot
GMA-741
Factor XIII
ELISA
GMA-751
Factor V
ELISA, inhibitory
GMA-752
Factor V
ELISA, inhibitory
GMA-753
Factor V
ELISA, western blot
GMA-754
Factor V
ELISA
GMA-755
Factor V
ELISA, inhibitory
GMA-756
Factor V
ELISA, inhibitory
GMA-762
Factor X light chain
Factor X
ELISA, western blot, inhibitory
GMA-763
Factor X light chain
Factor X
ELISA, western blot, inhibitory
GMA-767
Factor X/Xa
Factor X
ELISA
GMA-769
Factor X/Xa
Factor X
ELISA, western blot
GMA-780
Factor VIII
ELISA, western blot
GMA-781
Factor VIII
ELISA, inhibitory
GMA-792
Prothrombin
ELISA, western blot
GMA-793
Prothrombin
ELISA
Control Antigens

Control Antigens

All Products
Catalog #
Specificity
Antigen
Applications
GMA-630
BSA and peptide-BSA conjugates.
Bovine Serum Albumin Antibody
ELISA
GMA-640
Ovalbumin
ELISA
GMA-660
Bovine IgG
ELISA
GMA-721
Keyhole Limpet Hemocyanin (KLH)
ELISA

Not sure what you need?

Our expert team will guide you every step of the way.

Contact Us