Bovine Serum Albumin Antibody (BSA)

Green Mountain Antibodies internally developed a mouse monoclonal antibody against bovine serum albumin. The BSA antibody, GMA-630, binds BSA and BSA-conjugated peptides in solid phase ELISA. The monoclonal does not work in immunoblotting. The bovine serum albumin antibody is an IgG2b isotype and tests >98& pure by SDS-PAGE.

BSA (bovine serum albumin), also known as Fraction V, is a soluble 66.5 kDa protein derived from cows. Albumin makes up about half of the serum blood proteins and is often used as a protein concentration standard, stabilizer for enzymes or blocking agent in laboratory settings. BSA is often used as a conjugate for small molecules, such as peptides, to increase their size and confer properties more amenable for assays such as solid phase ELISAs.

In experiments where BSA-conjugates are used, rigorous experiments should include negative controls that demonstrate that responses are to the peptide of interest, and not the BSA portion of the molecule- the anti-BSA antibody can be used as part of this control set.

In a recent Octet experiment to determine affinity between a custom antibody and its antigen, signals were too low to be accurately interpreted, possibly due to steric issues around presentation of the peptide. To overcome this, a method was developed in which GMA-630 was used to capture a Bovine Serum Albumin-conjugated peptide and thus present the peptide in an orientation to be better bound by its antibody, increasing signal to measurable levels.

Applications

ELISA: Binds BSA and peptide-BSA in solid-phase ELISA.

Immunoblotting: Does not blot.

Antibody to BSA Formulation:
Lyophilized from a 1 mg/ml solution with 10 mM NaH2PO4 0.15 M NaCl, 1.0% (w/v) mannitol, pH 7.4. Concentration determined by absorbance measurement at 280 nm and using an extinction coefficient of 1.4 (E0.1%).

Storage: Aliquot and store at -20° C for prolonged periods. Avoid freeze/thaw cycles. Alternatively, bring to 0.01% (w/v) sodium azide and store at 4° C.

Antibody to BSA is produced via in vitro production methods, under serum free conditions, and purified by Protein G affinity chromatography. Custom conjugation is available. Products typically ship within 24 hours after receiving the purchase order.